TM 8-227 Methods For Medical Laboratory Technicians, 1951-08-03 TM 8-227 AFM 160-14 CONTENTS Paragraphs Page CHAPTER I. BASIC LABORATORY PROCEDURES. Section I. Introduction 1-2 1 II. Medical supplies 3-6 1 III. Laboratory reports 7-9 3 IV. The compound microscope 10-12 4 V. Manipulation of glassware 13-19 6 VI. Cleaning glassware 20-25 10 VII. Sterilization 26-31 12 VIII. Care of laboratory animals 32-38 16 IX. Animal experimentation 39-48 19 X. Collection and preparation of human blood samples 49-52 23 XI. Miscellaneous procedures: Useful laboratory recipes and methods 53-58 28 CHAPTER 2. EXAMINATION OF BLOOD. Section I. Clinical procedures 59-81 32 II. Human blood groups 82-101 78 CHAPTER 3. GENERAL CHEMICAL TECHNIC. Section I. Types of chemical analyses 102-103 98 II. The analytical balance 104-111 99 III. Volumetric glassware 112-115 105 Section IV. Filters and filtration 116-119 115 V. Acid-base indicators 120-123 120 VI. Oxidation-reduction reactions 124-126 132 VII. Volumetric titrations and solutions 127-145 136 VIII. Colorimetry 146-148 154 IX. Photometry 149-157 160 CHAPTER 4. EXAMINATION OF URINE. Section I. Collection and preservation of specimens 158 170 II. Physical examination 159 170 III. Microscopic examination of urinary sediments 160-163 171 IV. Qualitative chemical tests 164-169 174 V. Quantitative chemical analyses 170-178 178 CHAPTER 5. EXAMINATION OF GASTRIC CONTENTS. Section I. Physical examination 179-180 197 II. Tests of gastric secretion 181-182 197 III. Chemical analysis 183-186 199 CHAPTER 6. CHEMICAL ANALYSIS OF BLOOD AND SPINAL FLUID. Section I. Preparation of blood, plasma, and serum for quantitative chemical analysis 187-190 202 II. Chemical analysis 191-219 209 CHAPTER 7. GENERAL BACTERIOLOGIC METHODS. Section I. Collection of bacteriologic specimens 220-238 323 II. Microscopical and staining methods 239-254 329 III. Cultural methods 255-269 338 IV. General methods 270-276 351 V. Biochemical tests 277-296 379 VI. Serological methods 297-309 387 VII. Handling of bacteriological specimens 310-325 405 VIII. Preparation of autogenous vaccines 326-330 410 IX. Other typing and testing procedures 331-332 412 CHAPTER 8. CLASSIFICATION OF BACTERIA AND FUNGI. Section I. Description of bacteria 333-395 414 II. Mycology 396-402 490 CHAPTER 9. BACTERIAL FOOD POISONING 403-405 506 CHAPTER 10. WATER AND DAIRY BACTERIOLOGY. Section I. Bacteriologic examination of water 406-413 509 II. Bacteriologic examination of dairy products 414-416 517 CHAPTER II. RICKETTSIAE AND VIRUSES. Section I. Introduction 417-421 523 II. Rickettsiae 422-432 529 III. Viruses 433-442 539 CHAPTER 12. SERODIAGNOSIS OF SYPHILIS 443-447 559 CHAPTER 13. PROTOZOOLOGICAL METHODS. Section I. Protozoa 448 578 II. Intestinal protozoa 449-456 578 III. Flagellates of blood and other tissues 457-459 593 IV. Malarial parasites 460-465 602 CHAPTER 14. HELMINTHOLOGICAL METHODS. Section I. Classification 466-467 618 II. Important pathogenic species 468-472 621 III. Methods of examination 473-479 629 CHAPTER 15. ENTOMOLOGICAL METHODS. Section I. Distribution and classification of arthropods 480-481 635 II. Arthropods of medical importance 482-499 637 III. Collection, preparation, and shipment of specimens 500-508 662 CHAPTER 16. PATHOLOGIC METHODS. Section I. Gross pathology methods 509-516 670 II. Histological technic 517-520 677 III. Special stains 521-541 683 APPENDIX. REFERENCES 696 INDEX 700 Digitized by Google.

TM 8-227: AFM 160-14

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